Article
Protein kinase B/Akt phosphorylates and inhibits the cardiac Na+/H+ exchanger NHE1.
Circulation research - 10 Oct 2008
Snabaitis Andrew K, Cuello Friederike, Avkiran Metin
Abstract excerpt
Sarcolemmal Na(+)/H(+) exchanger (NHE) activity is mediated by NHE isoform 1 (NHE1), which is subject to regulation by protein kinases. Our objectives were to determine whether NHE1 is phosphorylated by protein kinase B (PKB), identify any pertinent phosphorylation site(s), and delineate the functional consequences of such phosphorylation. Active PKBalpha phosphorylated in vitro a glutathione S-transferase...
Topics
- Acidosis
- Animals
- Calmodulin
- Cation Transport Proteins
- Cells, Cultured
- Glycogen Synthase Kinase 3
- Glycogen Synthase Kinase 3 beta
- Humans
- Hydrogen-Ion Concentration
- Mutation
- Myocytes, Cardiac
- Phosphorylation
- Protein Kinase Inhibitors
- Proto-Oncogene Proteins c-akt
- Rats
- Recombinant Fusion Proteins
- Sarcolemma
- Serine