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Article

Conserved Glu40 and Glu433 of the biotin carboxylase domain of yeast pyruvate carboxylase I isoenzyme are essential for the association of tetramers.

The international journal of biochemistry & cell biology - 1 Jan 2007

Jitrapakdee Sarawut, Surinya Katharina H, Adina-Zada Abdussalam, Polyak Steven W, Stojkoski Cvetan, Smyth Roger, Booker Grant W, Cleland W Wallace, Attwood Paul V, Wallace John C

Abstract excerpt

The native form of pyruvate carboxylase is an alpha4 tetramer but the tetramerisation domain of each subunit is currently unknown. To identify this domain we co-expressed yeast pyruvate carboxylase 1 isozyme (Pyc1) with an N-terminal myc tag, together with constructs encoding either the biotin carboxylase (BC) domain or the transcarboxylase-biotin carboxyl carrier domain (TC-BCC), each with an N-terminal...

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PMID: 17659996
DOI: 10.1016/j.biocel.2007.06.006

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