Article
[Effect of Cry1Ca7 protein modified by site-directed mutagenesis on inhibiting Spodoptera exigua Hübner].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Jun 2008
Ren Yu, Liu Huamei, Song Fuping, Huang Dafang, Zhang Jie
Abstract excerpt
OBJECTIVE: To obtain the mutants with different toxicity from the wild-type CrylCa7. METHODS: Insecticidal crystal protein CrylCa7 from Bacillus thuringiensis which is highly toxic to Spodoptera exigua, an important agricultural pest in China, and we mutated this toxin by over-lapping extensive PCR method in different domains to obtain 11 chimeric mutants. RESULTS: The results of bioassays against Spodoptera...
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