Article
[Relationship between structure and function of loops from Bacillus thuringiensis insecticidal crystal protein Cry1Ba].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Sept 2008
Wang Guangjun, Zhang Jie, Sun Donghui, Song Fuping, Huang Dafang
Abstract excerpt
To indicate the relationship between structure and function of loops from Bacillus thuringiensis insecticidal crystal protein Cry1Ba, and the influence of amino acids mutation on toxicity against diamond back moth Plutella xylostella, five mutations at the loops of Cry1Ba were constructed by overlapping primer PCR, and expressed in E. coli BL21 (DE3). Bioassay results showed that the toxicity of mutation M1...
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