Article
[Identification of cry-type genes of 31 Bacillus thuringiensis isolates and analysis of their expression product].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Aug 2000
Zhang J, Song F, Zuo Y, Dai L, Huang D
Abstract excerpt
In this study, cry-type gene of 31 isolates of Bt had been identified using PCR-RFLP identification system, and SDS-PAGE analysis and bioassay had been performed. 25 strains containing cry1 type gene express 130-150 kD protein and 16 of them contain cry1I gene, which codes 80 kD protein and is larvicidal to both lepidopteran and coleopteran species. 15 strains contain both cry1 and cry2 type gene; 10 strains...
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