Article
Molecular characterization of a novel family-46 chitosanase from Pseudomonas sp. A-01.
Bioscience, biotechnology, and biochemistry - 1 Aug 2008
Ando Akikazu, Saito Akihiro, Arai Sayaka, Usuda Sakiko, Furuno Maiko, Kaneko Naomi, Shida Osamu, Nagata Yoshiho
Abstract excerpt
Pseudomonas sp. A-01, isolated as a strain with chitosan-degrading activity, produced a 28 kDa chitosanase. Following purification of the chitosanase (Cto1) and determination of its N-terminal amino acid sequence, the corresponding gene (cto1) was cloned by a reverse-genetic technique. The gene encoded a protein, composed of 266 amino acids, including a putative signal sequence (1-28), that showed an amino acid...
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