Article
Molecular characterization and antifungal activity of a family 46 chitosanase from Amycolatopsis sp. CsO-2.
FEMS microbiology letters - 1 Apr 2009
Saito Akihiro, Ooya Takaaki, Miyatsuchi Daisuke, Fuchigami Hiroko, Terakado Kanako, Nakayama Shin-ya, Watanabe Takeshi, Nagata Yoshiho, Ando Akikazu
Abstract excerpt
An actinomycete strain, Amycolatopsis sp. CsO-2, produces a 27-kDa chitosanase. To reveal the molecular characteristics of the enzyme, its corresponding gene ctoA was cloned by a reverse genetic technique, based on the N-terminal amino acid sequence of the protein. The encoded CtoA protein was deduced to be composed of 286 amino acids, including a putative signal peptide (1-48), and exhibited 83% identity in the...
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