Conserving, Distributing and Managing Genetically Modified Mouse Lines by Sperm Cryopreservation

Abstract excerpt

BACKGROUND: Sperm from C57BL/6 mice are difficult to cryopreserve and recover. Yet, the majority of genetically modified (GM) lines are maintained on this genetic background. METHODOLOGY/PRINCIPAL FINDINGS: Reported here is the development of an easily implemented method that consistently yields fertilization rates of 70+/-5% with this strain. This six-fold increase is achieved by collecting sperm from the vas...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Animal Genetics and Reproduction
• Share to Reproductive Biology and Fertility
• Share to Sperm and Testicular Function