Article
Phenotypic analysis of C57BL/6J and FVB/NJ mice generated using evaporatively dried spermatozoa.
Comparative medicine - 1 Oct 2007
Li Ming-Wen, Biggers John D, Toner Mehmet, Griffey Stephen M, Lloyd K C Kent
Abstract excerpt
Combination of evaporative drying and frozen storage at -80 degrees C has been used successfully to preserve hybrid B6D2F1 mouse spermatozoa. To determine whether this method can be applied equally well to inbred mice, spermatozoa of C57BL/6J and FVB/ NJ mice were evaporatively dried and stored for 1 mo at -80 degrees C before being used for intracytoplasmic sperm injection (ICSI) to produce live offspring. After...
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