Article
Long-term cryopreservation of mouse sperm.
Theriogenology - 15 Sept 2006
Kaneko Takehito, Yamamura Ayako, Ide Yukie, Ogi Mami, Yanagita Tomoko, Nakagata Naomi
Abstract excerpt
The objective was to determine if mouse sperm can maintain their fertilizing ability after being frozen for >10 y and whether the offspring derived from these sperm had normal fertilizing ability and phenotype. We cryopreserved sperm from six strains of mice (C57BL/6J, DBA/2N, BALB/cA, C3H/HeJ, B6D2F1 and B6C3F1) in a solution containing 18% (w/v) raffinose and 3% (w/v) skim milk, and preserved them in liquid...
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