Article
Identification of neural outgrowth genes using genome-wide RNAi.
PLoS genetics - 4 Jul 2008
Sepp Katharine J, Hong Pengyu, Lizarraga Sofia B, Liu Judy S, Mejia Luis A, Walsh Christopher A, Perrimon Norbert
Abstract excerpt
While genetic screens have identified many genes essential for neurite outgrowth, they have been limited in their ability to identify neural genes that also have earlier critical roles in the gastrula, or neural genes for which maternally contributed RNA compensates for gene mutations in the zygote. To address this, we developed methods to screen the Drosophila genome using RNA-interference (RNAi) on primary...
Topics
- Animals
- Cells, Cultured
- Drosophila
- Genome
- Genomics
- Mice
- Nervous System
- Neurons
- Phenotype
- RNA Interference
- RNA, Small Interfering
- ran GTP-Binding Protein