Article
The Cgl1281-encoding putative transporter of the cation diffusion facilitator family is responsible for alkali-tolerance in Corynebacterium glutamicum.
Archives of microbiology - 1 Nov 2008
Takeno Seiki, Nakamura Mio, Fukai Rie, Ohnishi Junko, Ikeda Masato
Abstract excerpt
Mutants of Corynebacterium glutamicum that were unable to grow under mild alkaline pH conditions were isolated by mutagenesis. Strain AL-43 exhibiting the highest sensitivity to alkaline pH among the mutants was selected and used to clone a DNA fragment that could complement the phenotype. Sequencing and subcloning of the cloned 4.0-kb EcoRI DNA fragment showed that the Cgl1281 gene was responsible for the...
Topics
- Adaptation, Physiological
- Alkalies
- Amino Acid Sequence
- Anti-Bacterial Agents
- Bacterial Proteins
- Cloning, Molecular
- Cobalt
- Corynebacterium glutamicum
- Drug Resistance, Bacterial
- Gene Order