Biochemical and genetic studies on the function of, and relationship between, the PGI1- and CDC30-encoded phosphoglucose isomerases in Saccharomyces cerevisiae.

Abstract excerpt

Isoelectric focusing was used to compare the complement of phosphoglucose isomerase isoenzymes in a wild-type strain of Saccharomyces cerevisiae and in a strain with a deletion in the PGI1 structural gene. Deletion of the PGI1 gene did not result in the absence of the high-Km isoenzyme I but the low-Km isoenzyme II was absent. Hence, the isoenzymes must be the products of two genes. If PGI1 were the sole...

Topics

• Chromosome Deletion
• Genes, Fungal
• Glucose
• Glucose-6-Phosphate Isomerase
• Isoelectric Focusing
• Isoenzymes
• Mutation
• Phenotype
• Saccharomyces cerevisiae
• Temperature