Physiological and morphological effects of genetic alterations leading to a reduced synthesis of UDP-glucose in Saccharomyces cerevisiae.

doi:10.1111/j.1574-6968.1997.tb10468.x

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Article

Physiological and morphological effects of genetic alterations leading to a reduced synthesis of UDP-glucose in Saccharomyces cerevisiae.

FEMS microbiology letters - 1 Aug 1997

Daran J M, Bell W, François J

Abstract excerpt

Yeast cells lacking UDP-Glc pyrophosphorylase (UGPase) encoded by UGPI are not viable. Two strategies were developed to drastically reduce the intracellular concentration of UDP-Glc in order to study the consequences of this metabolic engineering on physiology and morphology. Firstly, UGP1 was placed under the strongly regulatable THI4 promoter. This resulted in a 95% reduction of UGPase activity in the presence...

Topics

Cell Wall
Mutation
Phosphoglucomutase
Promoter Regions, Genetic
Recombinant Fusion Proteins
Saccharomyces cerevisiae
Thiamine
Trehalose
UTP-Glucose-1-Phosphate Uridylyltransferase
Uridine Diphosphate Glucose

Source

Open in PubMed

Identifiers

PMID: 9252577
DOI: 10.1111/j.1574-6968.1997.tb10468.x

Genes

CYC1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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