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Simple, rapid and sensitive detection of Orientia tsutsugamushi by loop-isothermal DNA amplification

19 Jun 2008

Abstract excerpt

We present a loop-mediated isothermal PCR assay (LAMP) targeting the groEL gene, which encodes the 60kDa heat shock protein of Orientia tsutsugamushi. Evaluation included testing of 63 samples of contemporary in vitro isolates, buffy coats and whole blood samples from patients with fever. Detection limits for LAMP were assessed by serial dilutions and quantitation by real-time PCR assay based on the same target...

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PMID: 18565558
DOI: 10.1016/j.trstmh.2008.04.040

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