Article

A multicopy <i>transposase</i> -targeted qPCR assay for highly sensitive diagnosis of scrub typhus

2026-04-02

Abstract excerpt

<h4>Objectives</h4> Scrub typhus, caused by the bacterium Orientia tsutsugamushi, is frequently underdiagnosed due to its non-specific clinical presentation and the frequent absence of eschar. Most molecular diagnostic assays target single-copy genes of O. tsutsugamushi , which can limit diagnostic sensitivity. We aimed to develop an ultra-sensitive quantitative PCR (qPCR) assay targeting a highly repetitive el...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 0c300d25-cf05-57db-abeb-b45323c6fc3c
DOI: 10.64898/2026.04.01.26349932

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

A multicopy <i>transposase</i> -targeted qPCR assay for highly sensitive diagnosis of scrub typhusDOI 10.64898/2026.04.01.26349932Read

Dual RNA-seq provides insight into the biology of the neglected intracellular human pathogen <i>Orientia tsutsugamushi</i>Semantic proximity Read

Evaluation of an in-house PCR for diagnosing scrub typhus along with a preliminary study of genotypic characterization of Orientia tsutshugamushi circulating in Chhattisgarh: A prospective clinico-microbiological study.Semantic proximity Read

Scrub Typhus in a Malaysian cohort: Epidemiological Trends, Clinical Outcomes, and Molecular DiversitySemantic proximity Read

A real-time PCR for the differentiation of typhoidal and non-typhoidal <i>Salmonella</i>Semantic proximity Read

Molecular Epidemiology and Genetic Diversity of Orientia tsutsugamushi From Patients and Small Mammals in Xiangyun County, Yunnan Province, ChinaSemantic proximity Read

Targeted sequence capture of <i>Orientia tsutsugamushi</i> DNA from chiggers and humansSemantic proximity Read

Comparison of Conventional, Nested, and Real-Time Quantitative PCR for Diagnosis of Scrub TyphusSemantic proximity Read

Developing a multiplex loop-mediated isothermal amplification assay (LAMP) to determine severe fever with thrombocytopenia syndrome (SFTS) and scrub typhusSemantic proximity Read

Development of a real-time PCR assay for the diagnosis of scrub typhus cases in India and evidence of the prevalence of new genotype of O. tsutsugamushi.Semantic proximity Read

Long-read whole genome sequencing and comparative analysis of six strains of the human pathogen <i>Orientia tsutsugamushi</i>Semantic proximity Read

Identification of Five NovelSalmonellaTyphi-Specific Genes as Markers for Diagnosis of Typhoid Fever Using Single-Gene Target PCR AssaysSemantic proximity Read

Multiplex PCR Assay to Detect High Risk Lineages of <i>Salmonella</i> Typhi and Paratyphi ASemantic proximity Read