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Comparison of Conventional, Nested, and Real-Time Quantitative PCR for Diagnosis of Scrub Typhus

11 Nov 2010

Abstract excerpt

Orientia tsutsugamushi is the causative agent of scrub typhus. For the diagnosis of scrub typhus, we investigated the performances of conventional PCR (C-PCR), nested PCR (N-PCR), and real-time quantitative PCR (Q-PCR) targeting the O. tsutsugamushi-specific 47-kDa gene. To compare the detection sensitivities of the three techniques, we used two template systems that used plasmid DNA (plasmid detection...

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PMID: 21068287
DOI: 10.1128/jcm.01216-09

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