Article
Development of a real-time PCR assay for the diagnosis of scrub typhus cases in India and evidence of the prevalence of new genotype of O. tsutsugamushi.
Acta tropica - 1 Oct 2007
Bakshi Diprabhanu, Singhal Pradeep, Mahajan Sanjay K, Subramaniam Prasanna, Tuteja Urmil, Batra Harsh Vardhan
Abstract excerpt
A qualitative syber green real-time PCR with primers designed for a truncated portion of the 56kDa major outer membrane antigen gene of Orientia tsutsugamushi was used to diagnose scrub typhus from the blood or serum of suspected patients. Sixty-six blood and/or sera samples from fever cases, either with high index of suspicion for scrub typhus and/or positive by Weil-Felix test (> or = 1:160), were tested with...
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