Article
Cloning, genetic analysis, and nucleotide sequence of a determinant coding for a 19-kilodalton peptidoglycan-associated protein (Ppl) of Legionella pneumophila.
Infection and immunity - 1 Aug 1991
Ludwig B, Schmid A, Marre R, Hacker J
Abstract excerpt
A genomic library of Legionella pneumophila, the causative agent of Legionnaires disease in humans, was constructed in Escherichia coli K-12, and the recombinant clones were screened by immuno-colony blots with an antiserum raised against heat-killed L. pneumophila. Twenty-three clones coding for...
Topics
- Amino Acid Sequence
- Bacterial Outer Membrane Proteins
- Base Sequence
- Blotting, Western
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Escherichia coli Proteins
- Haemophilus influenzae
- Legionella
- Lipoproteins
- Molecular Sequence Data
- Mutation
- Peptidoglycan
- Proteoglycans
- Restriction Mapping
- Sequence Homology, Nucleic Acid