Article
Primase-based whole genome amplification.
Nucleic acids research - 1 Aug 2008
Li Ying, Kim Hyun-Jin, Zheng Chunyang, Chow Wing Huen A, Lim Jeonghwa, Keenan Brendan, Pan Xiaojing, Lemieux Bertrand, Kong Huimin
Abstract excerpt
In vitro DNA amplification methods, such as polymerase chain reaction (PCR), rely on synthetic oligonucleotide primers for initiation of the reaction. In vivo, primers are synthesized on-template by DNA primase. The bacteriophage T7 gene 4 protein (gp4) has both primase and helicase activities. In this study, we report the development of a primase-based Whole Genome Amplification (pWGA) method, which utilizes gp4...
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