Article
Strategies and technical challenges in allele level Class II typing in 2578 bone marrow transplantation donor-recipient pairs.
Human immunology - 1 Jan 2000
Williams T M, Winden T, Setterholm M, Vierra-Green C A, Spellman S, Flesch S, Awdeh Z, Baxter-Lowe L A, Begovich A B, Fernandez-Vina M, Hegland J, Hurley C K, Johnson D, Noreen H, Salazar M, Schmeckpeper B, Yunis E J
Abstract excerpt
Human leukocyte antigen typing of 2578 donor-recipient pairs whose transplantation was facilitated by the National Marrow Donor Program allowed for an in-depth analysis of the accuracy of high-volume allele level testing data. The methods employed provided allele level typing at DRB1/3/5, DQA1, DQB1, DPA1, and DPB1 using sequence-specific oligonucleotide probe hybridization (SSOPH), polymerase chain reaction...
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