Article
Efficient, automatic detection of heterozygous bases during large-scale DNA sequence screening.
BioTechniques - 1 Dec 1995
Phelps R S, Chadwick R B, Conrad M P, Kronick M N, Kamb A
Abstract excerpt
A crucial factor in the success of positional cloning efforts is the ability to screen rapidly many different candidate genes for mutations. By modifying standard software, we have improved the detection of heterozygous base positions in PCR products sequenced by cycle sequencing. A key element of the method is the incorporation of a modified heterozygote detection algorithm that permits the use of DNA sequence...
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