Article
PCR methods of genotyping.
Current protocols in human genetics - 1 May 2001
Hudson T J, Clark C D, Gschwend M, Justice-Higgins E
Abstract excerpt
Two protocols discuss labeling PCR products with radioactive labels. The PCR products are then analyzed on a denaturing polyacrylamide gel and visualized by direct autoradiography. The resulting band pattern is used to define the SSLP genotype of the individual. Another method of genotyping uses a simple silver staining technique to detect PCR-amplified SSLPs electrophoresed in denaturing polyacrylamide gels. In...
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