Article
RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance.
Development (Cambridge, England) - 1 Apr 2008
Bai Jianwu, Binari Richard, Ni Jian-Quan, Vijayakanthan Marina, Li Hong-Sheng, Perrimon Norbert
Abstract excerpt
To facilitate the genetic analysis of muscle assembly and maintenance, we have developed a method for efficient RNA interference (RNAi) in Drosophila primary cells using double-stranded RNAs (dsRNAs). First, using molecular markers, we confirm and extend the observation that myogenesis in primary cultures derived from Drosophila embryonic cells follows the same developmental course as that seen in vivo. Second,...
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