Functionally modified chitotriosidase catalytic domain for chitin detection based on split-luciferase complementation.

Abstract excerpt

In this study, we applied a luciferase-fragment complementation assay for chitin detection. When luciferase-fragment fused chitin-binding proteins were mixed with chitin, the reconstituted luciferase became active. The recombinant chitin-binding domain (CBD) and a functionally modified catalytic domain (CatD) of human chitotriosidase were employed for this method. We designed the CatD mutant as a chitin-binding...

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• Biosensing Techniques
• Candida albicans
• Carbohydrates
• Catalytic Domain
• Chitin