Article
High-resolution NMR analysis of the conformations of native and base analog substituted retroviral and LTR-retrotransposon PPT primers.
Chemistry & biology - 1 Mar 2008
Yi-Brunozzi Hye Young, Brinson Robert G, Brabazon Danielle M, Lener Daniela, Le Grice Stuart F J, Marino John P
Abstract excerpt
A purine-rich region of the plus-strand RNA genome of retroviruses and long terminal repeat (LTR)-containing retrotransposons, known as the polypurine tract (PPT), is resistant to hydrolysis by the RNase H domain of reverse transcriptase (RT) and ultimately serves as a primer for plus-strand DNA synthesis. The mechanisms underlying PPT resistance and selective processing remain largely unknown. Here, two RNA/DNA...
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