Functional dissection in vitro of the human c-fos promoter.

Abstract excerpt

Using internal and 5' deletions, the elements contributing to the in vitro activity of the human c-fos promoter have been identified. Wild type and mutant promoters were fused to the G-free cassette and tested, using HeLa nuclear and whole cell extracts, with the fos wild type promoter as the internal control. The proximal promoter domain, spanning from -124 to -58 in the fos promoter, is the primary determinant...

Topics

• Activating Transcription Factors
• Base Sequence
• Blood Proteins
• Cyclic AMP Response Element-Binding Protein
• DNA-Binding Proteins
• HeLa Cells
• Humans
• Molecular Sequence Data
• Mutation
• Nuclear Proteins
• Promoter Regions, Genetic