Article
Screens for mitochondrial mutants in yeast.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2007
Foury Françoise
Abstract excerpt
We describe methods that allow isolation, identification, and counting of mitochondrial mutants that are resistant to antibiotics (ant(R)) or respiratory deficient (rho-). (1) Analysis of diploid and meiotic progenies generated in crosses between mutants and tester strains allows distinguishing nuclear from mitochondrial mutants, for either antibiotic resistance or respiratory deficiency. (2) The mutation rate of...
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