Article
Deletion of many yeast introns reveals a minority of genes that require splicing for function.
Molecular biology of the cell - 1 May 2008
Parenteau Julie, Durand Mathieu, Véronneau Steeve, Lacombe Andrée-Anne, Morin Geneviève, Guérin Valérie, Cecez Bojana, Gervais-Bird Julien, Koh Chu-Shin, Brunelle David, Wellinger Raymund J, Chabot Benoit, Abou Elela Sherif
Abstract excerpt
Splicing regulates gene expression and contributes to proteomic diversity in higher eukaryotes. However, in yeast only 283 of the 6000 genes contain introns and their impact on cell function is not clear. To assess the contribution of introns to cell function, we initiated large-scale intron deletions in yeast with the ultimate goal of creating an intron-free model eukaryote. We show that about one-third of yeast...
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