Article
Physical and genetic analysis of the phosphoenolpyruvate carboxykinase (pckA) locus from Escherichia coli K12.
Molecular & general genetics : MGG - 1 Jan 1990
Goldie H, Medina V
Abstract excerpt
An 8 kb BamHI fragment of the Escherichia coli K12 chromosome has been cloned which complemented the pheotype of CRM+ pckA mutants with inactive phosphoenolpyruvate (PEP) carboxykinase. The pckA+ clones expressed levels of enzyme activity elevated up to 30-fold and produced a Mr 55,000 product in maxicells, which co-electrophoresed with purified PEP carboxykinase. The cloned fragment expressed the pckA, ompR and...
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