Physical and genetic analysis of the phosphoenolpyruvate carboxykinase (pckA) locus from Escherichia coli K12.

doi:10.1007/BF00260481

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Article

Physical and genetic analysis of the phosphoenolpyruvate carboxykinase (pckA) locus from Escherichia coli K12.

Molecular & general genetics : MGG - 1 Jan 1990

Goldie H, Medina V

Abstract excerpt

An 8 kb BamHI fragment of the Escherichia coli K12 chromosome has been cloned which complemented the pheotype of CRM+ pckA mutants with inactive phosphoenolpyruvate (PEP) carboxykinase. The pckA+ clones expressed levels of enzyme activity elevated up to 30-fold and produced a Mr 55,000 product in...

Topics

Carboxy-Lyases
Chloramphenicol O-Acetyltransferase
Chromosome Mapping
Chromosomes, Bacterial
Cloning, Molecular
Escherichia coli
Genes, Bacterial
Genotype
Phosphoenolpyruvate Carboxylase
Plasmids
Restriction Mapping
Transcription, Genetic
Transformation, Bacterial

Source

Open in PubMed

Identifiers

PMID: 2183002
DOI: 10.1007/BF00260481

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2183002-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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