Article
Accumulating variation at conserved sites in potyvirus genomes is driven by species discovery and affects degenerate primer design.
PloS one - 13 Feb 2008
Zheng Linda, Wayper Paul J, Gibbs Adrian J, Fourment Mathieu, Rodoni Brendan C, Gibbs Mark J
Abstract excerpt
UNLABELLED: Unknown and foreign viruses can be detected using degenerate primers targeted at conserved sites in the known viral gene sequences. Conserved sites are found by comparing sequences and so the usefulness of a set of primers depends crucially on how well the known sequences represent the target group including unknown sequences. METHODOLOGY/PRINCIPAL FINDINGS: We developed a method for assessing the...
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