Article
PCR bias associated with conserved primer binding sites, used to determine genotype diversity within Citrus tristeza virus populations.
Journal of virological methods - 1 Nov 2016
Read David Alan, Pietersen Gerhard
Abstract excerpt
Citrus tristeza virus (CTV) is present in almost all of the major citrus production areas where it continues to reduce the profitability of citriculture. The accurate characterisation of CTV populations, which are usually made up of a number of disparate strains, requires the use of robust PCR protocols. Mismatches between primers and their corresponding binding sites may introduce primer-associated bias during...
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