Article
A rapid and accurate approach to identify single nucleotide polymorphisms of mitochondrial DNA using MALDI-TOF mass spectrometry.
Clinical chemistry and laboratory medicine - 1 Jan 2008
Xiu-Cheng Fan Alex, Garritsen Henk S P, Tarhouny Shereen E L, Morris Michael, Hahn Sinuhe, Holzgreve Wolfgang, Zhong Xiao Yan
Abstract excerpt
BACKGROUND: Single nucleotide polymorphisms (SNPs) of mitochondrial DNA (mtDNA) are involved in physiological and pathological conditions. We developed a rapid, accurate, highly sensitive and high-throughput approach with low cost to identify mtDNA SNPs. METHODS: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to detect 18 SNPs of mtDNA by uniplex and multiplex...
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