Article
A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis.
Journal of microbiological methods - 1 Jun 2008
Kenefic L J, Beaudry J, Trim C, Huynh L, Zanecki S, Matthews M, Schupp J, Van Ert M, Keim P
Abstract excerpt
The allelic identities of Single Nucleotide Repeat (SNR) markers in Bacillus anthracis are typically ascertained by DNA sequencing through the direct repeat. Here we describe a reproducible method for genotyping closely related isolates by using four SNR loci in a multiplex-PCR capillary electrophoresis system amenable to high-throughput analysis.
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