Article
Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis.
Journal of clinical microbiology - 1 Apr 2005
Easterday W Ryan, Van Ert Matthew N, Simonson Tatum S, Wagner David M, Kenefic Leo J, Allender Christopher J, Keim Paul
Abstract excerpt
A TaqMan-minor groove binding assay designed around a nonsense mutation in the plcR gene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates. The assay differentiated B. anthracis from these genetic near-neighbors and determined that the nonsense mutation is ubiquitous across 89 globally and genetically diverse B. anthracis strains.
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