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Article

New methodological approach to induce a differentiation phenotype in Caco-2 cells prior to post-confluence stage.

Anticancer research - 1 Jan 2000

Ferraretto Anita, Gravaghi Claudia, Donetti Elena, Cosentino Stefania, Donida Bianca M, Bedoni Marzia, Lombardi Giovanni, Fiorilli Amelia, Tettamanti Guido

Abstract excerpt

BACKGROUND: Various differentiation-inducing agents or harvesting of spontaneously late post-confluence cultures have been used to differentiate the human colon carcinoma Caco-2 cell line. We report a new procedure to generate pre-confluent subcultures of Caco-2 population at various stages of differentiation without altering culture conditions. MATERIALS AND METHODS: Ultrastructural analysis, cell proliferation...

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PMID: 18225551

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