Article
Mutation in the Escherichia coli htpR locus results in stabilization of recombinant expression products that are susceptible to proteolytic degradation.
Protein expression and purification - 1 Jan 2000
Lindler L E, Anders J C, Herman W E
Abstract excerpt
We compared the expression and degradation of three cloned malarial proteins in a pair of isogeneic strains of Escherichia coli that differed at the htpR locus. The htpR locus encodes an alternate sigma factor necessary for the transcription of heat shock promoters. Plasmodium sequences were clon...
Topics
- Amino Acid Sequence
- Animals
- Antigens, Protozoan
- Base Sequence
- Cloning, Molecular
- DNA, Protozoan
- DNA-Directed RNA Polymerases
- Escherichia coli
- Gene Expression
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Peptide Hydrolases
- Plasmodium
- Protozoan Proteins
- Recombinant Proteins
- Sigma Factor