Article
High-throughput genotyping with infrared fluorescence allele specific hybridization (iFLASH): a simple, reliable and low-cost alternative.
Clinical biochemistry - 1 Jul 2006
van Himbergen Thomas M, Voorbij Hieronymus A M, Barendrecht Arjan D, van Rijn Bas B, Brambilla Roberta, van Tits Lambertus J H, Roest Mark
Abstract excerpt
OBJECTIVES: To develop and validate a novel genotyping approach, named infrared Fluorescence Allele Specific Hybridization (iFLASH), which combines the principles of allele specific oligonucleotide (ASO) hybridization with the advanced possibilities of infrared imaging. DESIGN AND METHODS: As an example, we genotyped the 55L > M and the 192Q > R common genetic variants of the paraoxonase-1 gene in 92 DNA samples...
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