Article
Molecular screening of canine GM1 gangliosidosis using blood smear specimens after prolonged storage: detection of carriers among shiba dogs in northern Japan.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc - 1 Jan 2008
Yamato Osamu, Jo Eun-Og, Chang Hye-Sook, Satoh Hiroyuki, Shoda Toru, Sato Reeko, Uechi Masami, Kawasaki Naomi, Naito Yoshihisa, Yamasaki Masahiro, Maede Yoshimitsu, Arai Toshiro
Abstract excerpt
Molecular screening of GM1 gangliosidosis in Shiba dogs was carried out in northern Japan using blood smear specimens after prolonged storage. Of 125 specimens obtained from 3 veterinary teaching hospitals for this screening, 68 specimens (54%) were adequate for direct amplification in a polymerase chain reaction (PCR)-based DNA test, and the percentage of adequacy was different at each hospital (34%, 73%, and...
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