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Article

Ultrafast excited-state dynamics in the green fluorescent protein variant S65T/H148D. 3. Short- and long-time dynamics of the excited-state proton transfer.

Biochemistry - 30 Oct 2007

Leiderman Pavel, Genosar Liat, Huppert Dan, Shu Xiaokun, Remington S James, Solntsev Kyril M, Tolbert Laren M

Abstract excerpt

Steady-state emission, femtosecond pump-probe spectroscopy, and time-correlated single-photon counting (TCSPC) measurements were used to study the photophysics and the excited-state proton transfer (ESPT) reactions in the green fluorescent protein (GFP) variant S65T/H148D at three pH values: 6.0, 7.9, and 9.5. Selective mutation of GFP caused a dramatic change in the steady-state and excited-state behavior as...

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PMID: 17918961
DOI: 10.1021/bi7009053

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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