Phage-enzymes: expression and affinity chromatography of functional alkaline phosphatase on the surface of bacteriophage.

doi:10.1093/protein/4.8.955

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Article

Phage-enzymes: expression and affinity chromatography of functional alkaline phosphatase on the surface of bacteriophage.

Protein engineering - 1 Dec 1991

McCafferty J, Jackson R H, Chiswell D J

Abstract excerpt

We have demonstrated that an active enzyme can be expressed on the surface of a bacteriophage. The gene encoding alkaline phosphatase from Escherichia coli was cloned upstream of gene 3, which encodes a minor coat protein of the filamentous bacteriophage, fd. A fusion protein of the correct size was detected from viral particles by Western blotting. Ultrafiltration confirmed that the enzyme fusion behaves as part...

Topics

Alkaline Phosphatase
Amino Acid Sequence
Bacteriophages
Base Sequence
Chromatography, Affinity
Escherichia coli
Gene Expression Regulation, Viral
Kinetics
Molecular Sequence Data
Mutation
Recombinant Fusion Proteins

Viral Envelope Proteins

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