Article
Mutations affecting the activity of urokinase-type plasminogen activator.
Protein engineering - 1 Dec 1991
Davidow L S, Dumais D R, Smyth A P, Greer J, Moir D T
Abstract excerpt
Mutagenesis throughout the single-chain urokinase-type plasminogen activator (scu-PA) cDNA molecule, followed by expression of the mutant genes and secretion of the resulting mutant proteins from yeast, has been used to determine the amino acid residues important for activity of scu-PA molecules. Twelve out of 13 colonies secreting variant scu-PA molecules with decreased ability to form a zone of fibrinolysis had...
Topics
- Base Sequence
- Binding Sites
- Down-Regulation
- Escherichia coli
- Gene Expression Regulation, Fungal
- Gene Library
- Genetic Vectors
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis
- Mutation