Article
Developmental fate determination and marker discovery in hematopoietic stem cell biology using proteomic fingerprinting.
Molecular & cellular proteomics : MCP - 1 Mar 2008
Spooncer Elaine, Brouard Nathalie, Nilsson Susie K, Williams Brenda, Liu Mira C, Unwin Richard D, Blinco David, Jaworska Ewa, Simmons Paul J, Whetton Anthony D
Abstract excerpt
In hematopoiesis, co-expression of Sca-1 and c-Kit defines cells (LS(+)K) with long term reconstituting potential. In contrast, poorly characterized LS(-)K cells fail to reconstitute lethally irradiated recipients. Relative quantification mass spectrometry and transcriptional profiling were used to characterize LS(+)K and LS(-)K cells. This approach yielded data on >1200 proteins. Only 32% of protein changes...
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