Article
Numerical evaluation of the invasion of closely related cell lines into collagen type I gels.
Invasion & metastasis - 1 Jan 1991
Vakaet L, Vleminckx K, Van Roy F, Mareel M
Abstract excerpt
Cells were seeded on top of a reconstituted collagen gel layer, and their migration into the gel was evaluated as an assay for invasive behavior. The method was standardized by measuring the depth of migration of each cell in a defined volume of the gel. We developed a microscope stage, controlled by a computer program. This semiautomatic counting method allowed precise vertical localization of each cell in a...
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