Article
Cftr gene targeting in mouse embryonic stem cells mediated by Small Fragment Homologous Replacement (SFHR).
Frontiers in bioscience : a journal and virtual library - 1 Jan 2008
Sangiuolo Federica, Scaldaferri Maria Lucia, Filareto Antonio, Spitalieri Paola, Guerra Lorenzo, Favia Maria, Caroppo Rosa, Mango Ruggiero, Bruscia Emanuela, Gruenert Dieter C, Casavola Valeria, De Felici Massimo, Novelli Giuseppe
Abstract excerpt
Different gene targeting approaches have been developed to modify endogenous genomic DNA in both human and mouse cells. Briefly, the process involves the targeting of a specific mutation in situ leading to the gene correction and the restoration of a normal gene function. Most of these protocols with therapeutic potential are oligonucleotide based, and rely on endogenous enzymatic pathways. One gene targeting...
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