Article
Targeted replacement of normal and mutant CFTR sequences in human airway epithelial cells using DNA fragments.
Human molecular genetics - 1 Nov 1998
Goncz K K, Kunzelmann K, Xu Z, Gruenert D C
Abstract excerpt
Recent studies have reported that mutant genomic cystic fibrosis (CF) transmembrane conductance regulator ( CFTR ) sequences can be corrected in transformed CF airway epithelial cell lines by targeted replacement with small fragments of DNA with wild-type sequence. To determine if the observed ge...
Topics
- Binding Sites
- Cell Line, Transformed
- Cells, Cultured
- Cystic Fibrosis
- Cystic Fibrosis Transmembrane Conductance Regulator
- DNA
- Deoxyribonucleases, Type II Site-Specific
- Epithelial Cells
- Eukaryotic Cells
- Gene Targeting
- Humans
- Mutation
- RNA
- Respiratory System
- Reverse Transcriptase Polymerase Chain Reaction