Article
Mechanical isolation of the inner cell mass is effective in derivation of new human embryonic stem cell lines
18 Oct 2007
Abstract excerpt
BACKGROUND: For clinical grade human embryonic stem cell (hESC) lines, a robust derivation system without any substances having animal origin would be required. We have gradually improved our hESC derivations. Human skin fibroblasts were used as feeder cells in derivation of all our 25 permanent fully characterized hESC lines. In the first four derivations, fetal calf serum was used as a supplement in the medium,...
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