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Serum-free medium cultivation to improve efficacy in establishment of human embryonic stem cell lines

2 Sept 2005

Abstract excerpt

BACKGROUND: Serum-containing and serum-free media were used to derive human embryonic stem (HES) cells from donated oocytes and embryos. METHODS AND RESULTS: Inner cell masses (ICM) were isolated by immunosurgery. The HES cells were found to be easily obtained and expanded in a serum-free medium. The efficacy in establishing human embryonic stem cell lines improved in a serum-free medium compared with that in...

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PMID: 16143641
DOI: 10.1093/humrep/dei275

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