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Article

Derivation of hESC from Intact Blastocysts

1 Jun 2007

Abstract excerpt

This unit describes protocols for culturing human embryos and deriving human embryonic stem cells from the intact blastocyst. Description of the culturing begins with methods for obtaining human blastocysts using pronuclear or cleavage stage embryos left over after in vitro fertilization. Then there is a description of methods that can be used to derive human embryonic stem cell lines from the blastocyst without...

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PMID: 18785158
DOI: 10.1002/9780470151808.sc01a02s1

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