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Derivation of 30 human embryonic stem cell lines—improving the quality

2 Mar 2010

Abstract excerpt

We have derived 30 human embryonic stem cell lines from supernumerary blastocysts in our laboratory. During the derivation process, we have studied new and safe method to establish good quality lines. All our human embryonic stem cell lines have been derived using human foreskin fibroblasts as feeder cells. The 26 more recent lines were derived in a medium containing serum replacement instead of fetal calf serum....

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PMID: 20198446
DOI: 10.1007/s11626-010-9308-0

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